Histone H1° is distributed unlike H1 in chromatin aggregation
نویسندگان
چکیده
منابع مشابه
Histone H1 gets Pin’d onto chromatin
Histone H1 gets Pin'd onto chromatin T he proline isomerase Pin1 limits chromosome decon-densation by stabilizing a histone's association with chromatin, Raghuram et al. reveal. Histone H1 incorporates into nucleosomes to enhance chromatin folding and condensation. Phosphory-lation by the cyclin-dependent kinase Cdk2 promotes H1's dissociation from chromatin, perhaps by altering the conformatio...
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The relative amount of H1 histone associated with isolated nucleosomes from calf thymus was determined as a function of the extent of DNA digestion by micrococcal nuclease. Generally the amount of H1 histone associated with mononucleosomes decreases with increasing digestion until 60% of the original H1 remains associated with DNA 150 base pirs or less in size. Coincidentally, H1 histone increa...
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Cellular differentiation during embryogenesis involves complex gene regulation to enable the activation and repression of genes. Here, we show that mesodermal competence is inhibited in Xenopus embryos depleted of histones H3 and H3.3, which fail to respond to Nodal/Activin signaling and exhibit concomitant loss of mesodermal gene expression. We find that transcriptional activation in gastrula ...
متن کاملExchange of H1 histone depends on aggregation of chromatin, not simply on ionic strength.
Chromatin solubility was observed at several concentrations of various cations. Spermine and spermidine precipitated (50%) chromatin at about 0.2 mM, Ca2+ and Mg2+ at about 1-2 mM, and Na+ at about 100 mM. Further increases in cation concentration induced more aggregation, but eventually excess cation increased chromatin solubility so that 50% solubility was observed again at 60 mM Mg2+ and 180...
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Histone H1 binds to linker DNA between nucleosomes, but the dynamics and biological ramifications of this interaction remain poorly understood. We performed single-molecule experiments using magnetic tweezers to determine the effects of H1 on naked DNA in buffer or during chromatin assembly in Xenopus egg extracts. In buffer, nanomolar concentrations of H1 induce bending and looping of naked DN...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1985
ISSN: 0014-5793
DOI: 10.1016/0014-5793(85)80353-7